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Antibodies certain to the blocking peptide no longer bind to your epitope within the target protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is precise.
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This tutorial outlines additional doable optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
This guide outlines even more probable optimization of cellular and immunoassay parameters to be sure the very best results are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind precisely for the goal antibody and block antibody binding. These peptide typically contains the epitope recognized by the antibody.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which contribute to your temporal coordination of each mitotic occasion. Cyclin E varieties get more info a posh with and functions being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates for the G1-S section boundary and is degraded as cells progress by way of S section.
Overexpression of this gene is noticed in lots of tumors, which leads to chromosome instability, and so may perhaps contribute to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential role in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two added splice variants were being noted but in-depth nucleotide sequence info will not be yet obtainable.
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